The United States Botanic Garden (USBG) contracted RK&A to explore visitors’ interest in and understanding of the design and interpretation presented in four conservatory rooms—Rare and Endangered Species, Plant Adaptations, Medicinal Plants, and Garden Primeval. Two rooms were quite small and the other two rooms were larger. Each room was designed to support a theme and included a variety of signage to support the theme (e.g., plant ID signs, ephemeral signs, thematic signs). Findings from this study will be used in part to inform a new interpretive strategy for the Garden. How did we